产品货号：
Z33877
中文名称：
RPA1抗体
英文名称：
Anti-RPA1 Antibody (Clone#11H4)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-RPA1 Antibody (Clone#11H4) |
| 指标别名 | HSSB;MST075;REPA1;Replication factor A protein 1;replication protein A1;70kDa;RF A;RF A protein 1;RP A;RP A p70;RPA1;RPA70 |
| 克隆性 | Monoclonal |
| 检验物种 | human,monkey,mouse |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | RPA1 |
| 克隆号 | 11H4 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2b |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human RPA70 (533-568aa QESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFR),different from the related mouse sequence by three amino acids. |
| 实际分子量 | 72KD |
| 成分 | 200μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 200μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Replication protein A 70kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.This gene is mapped to chromosome 17p13.3.Replication protein A (RPA) is a heterotrimeric single-strand DNA (ssDNA)-binding protein essential for DNA replication,repair,and recombination.It is composed of 70-kD (RPA1),32-kD (RPA2),and 14-kD (RPA3) subunits.The RPA1 subunit is responsible for high-affinity ssDNA binding.The RPA complex was originally isolated as a factor essential for in vitro replication of the papovavirus SV40.It had been found that recombinant human RPA1,purified from bacteria,exhibited ssDNA-binding activity comparable to that of the complete RPA complex.RPA1 could substitute for the complete complex in stimulating the activity of DNA polymerase alpha-primase,but it could not substitute for the complete complex in SV40 DNA replication in vitro,suggesting an important functional role for the other subunits. |
| Uniprot ID | P27694 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
相关搜索：RPA1抗体，RPA1单抗，Anti-RPA1 Antibody (Clone#11H4)